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constrained iterative deconvolution tool  (Softworx Inc)

 
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    Softworx Inc constrained iterative deconvolution tool
    Constrained Iterative Deconvolution Tool, supplied by Softworx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/constrained iterative deconvolution tool/product/Softworx Inc
    Average 90 stars, based on 1 article reviews
    constrained iterative deconvolution tool - by Bioz Stars, 2026-04
    90/100 stars

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    Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Journal: American journal of respiratory cell and molecular biology

    Article Title: Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen Challenged Mice

    doi: 10.1165/rcmb.2004-0060OC

    Figure Lengend Snippet: Mucin is produced by Clara cells in antigen challenged mice. Top. Airways from antigen challenged mice were stained immunohistochemically for CCSP and counterstained with either PAS (right) or alcian blue to stain acidic and sulfated mucins (left) to stain neutral mucins. In antigen challenged mice, alcian blue or PAS mucin granule staining colocalizes exclusively to cells immunoabeled positively for CCSP. Bottom. CCSP and mucin are packaged in the same secretory granules. Colocalization of mucin (red PAFS staining) and CCSP (blue CCSP immunolabeling) was determined by deconvolution microscopy in 0.2 μm optical planes. Pink in the merged image demonstrates colocalization of CCSP and PAFS within apical secretory granules. Scale bar is 10 μm in PAS stained images and 6 μm in alcian blue and PAFS stained images.

    Article Snippet: Tissues were observed on an inverted microscope by focusing on 45 consecutive 0.2 μm focal planes followed by constrained-iterative deconvolution (DeltaVision, Applied Precision, LLC, Issaquah, Washington).

    Techniques: Produced, Staining, Immunolabeling, Microscopy